Genomic analysis of Shiga toxin-producing Escherichia coli belonging novel O serogroups

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460540
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: Iguchi Atsushi 宮崎大学, 農学部, 准教授 (00437948)
• Co-Investigator(Renkei-kenkyūsha): IYODA SUNAO 国立感染症研究所, 細菌第一部, 主任研究官 (70300928) ; OHNISHI MAKOTO 国立感染症研究所, 細菌第一部, 部長 (10233214) ; SETO KAZUKO 大阪府立公衆衛生研究所, 細菌課, 主任研究員 (70211323)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 志賀毒素産生性大腸菌 / O血清群 / PCR

Outline of Final Research Achievements

We conducted genomic analysis of Shiga toxin-producing Escherichia coli belonging novel O serogroups (O182-O187). Based on the O-antigen biosynthesis gene clusters extracted from draft genomes, each O-serogroup-specific PCR method was designed. Screening by using the PCR methods showed that two O182, six O183, one O185 and one O186 were identified from 2014-2015 STEC isolates in Japan.

Free Research Field

細菌学