2015 Fiscal Year Final Research Report
Importance of Specification of RNA export pathway for HIV-1 gene expression
| Project/Area Number |
25460564
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ヒト免疫不全ウイルス / RNA核外輸送 / RNAスプライシング |
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| Outline of Final Research Achievements |
Although intron-containing HIV-1 RNAs have the same features as cellular general mRNAs, HIV-1 blocks the utilization of mRNA export factor, TAP, during nuclear RNA export. The blockage was achieved by the activity of viral Rev protein to inhibit TAP recruitment to viral RNAs. However, why Rev inhibits the recruitment remains a mystery.
Since viral RNAs are exported without being spliced, Rev may block TAP recruitment to escape the commitment to RNA splicing. I hypothesized that TAP stimulates RNA splicing.
To test the hypothesis, we performed in vitro splicing assay. Splicing products were increased when purified recombinant TAP protein was added. Conversely, RNA splicing was delayed by TAP inhibitor. These results support the hypothesis that TAP stimulates RNA splicing.
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| Free Research Field |
遺伝子発現制御
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