Analysis of formation of Aichi virus replication complexes

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460572
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Fujita Health University
• Principal Investigator: Sasaki Jun 藤田保健衛生大学, 医学部, 講師 (70319268)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アイチウイルス / ピコルナウイルス / RNAウイルス / ゲノム複製 / 宿主因子

Outline of Final Research Achievements

We have analyzed the machanism of genome replication of Aichi virus, a picornavirus associated with acute gastroenteritis in humans. In this study, we identified novel host factors required for Aichi virus genome replication. In addition, we revealed involvement of lipids in genome replication. At replication sites of Aichi virus genome, viral 2B, 2BC, 2C, 3A, and 3AB proteins and multiple host proteins were assembled through protein-protein interactions, resulting in accumulation of two kinds of lipid, PI4P and cholesterol. Moreover, accumulation of these lipids was indicated to be essential for Aichi virus genome replication.

Free Research Field

ウイルス学