Clathrin Heavy chain is a potential biomarker for histological diagnosis of various cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460675
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: SEIMIYA Masanori 千葉大学, 医学部附属病院, 副臨床検査技師長 (20554265)
• Co-Investigator(Kenkyū-buntansha): NOMURA Fumio 千葉大学, 医学部附属病院, 特任教授 (80164739) ; MATSUSHITA Kazuyuki 千葉大学, 大学院医学研究院, 准教授 (90344994)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 臨床検査 / 病理診断 / クラスリン重鎖 / 免疫組織化学

Outline of Final Research Achievements

Clathrin Heavy Chain (CHC) is an endocytosis related protein. We previously reported the immunohistochemical staining of CHC as a histological diagnostic marker of liver cancer or esophageal cancer. In this study, the usefulness for the histopathological diagnosis of pharynx carcinoma, larynx carcinoma, tongue carcinoma, and gingiva carcinoma were investigated. CHC was significantly more strongly expressed in the cytoplasm of malignant legions, than in that of normal tissue or benign lesions. Therefore, the immunohistochemical staining of CHC is a potential biomarker for histological diagnosis of these cancer. Moreover, we synthesized the 12 peptide for serum anti-CHC antibody level. It will be necessary to develop the ELISA method and to evaluate performance for diagnosis of various cancer.

Free Research Field

臨床検査学