Analysis of mRNA level of the treatment resistance related proteins in unfixed lung cancer biopsy specimens obtained by core-biopsy.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460691
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Nagasaki University
• Principal Investigator: NAKAMURA Yoichi 長崎大学, 医歯薬学総合研究科(医学系), 講師 (20432974)
• Co-Investigator(Kenkyū-buntansha): NAGASHIMA Seiji 長崎大学, 病院(医学系), 助教 (00646672) ; FUKUDA Minoru 長崎大学, 病院(医学系), 准教授 (50388930)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 非小細胞肺癌 / 個別化治療

Outline of Final Research Achievements

Total mRNAs were extracted from unfixed lung cancer biopsy specimens, and each RNA level was analyzed. They are compared as ΔCT. ABC transporters; Overexpression was shown in MDR1, MRP1, BCRP. Cytoskeleton proteins; Overexpression was shown in TUBB3, and mild down regulation was shown in Gelsolin. Vascular endothelial growth factors: mild overexpression was shown in VEGF-A and VEGF-B, and overexpression was shown in VEGF-C. Cancer stem cell related antigens: Overexpression was shown in CD20, POU5F1, PROM1, Nanog, KLF4, Notch1, but CD24 and CD44 were no change.

Free Research Field

呼吸器内科