Study of circulating microRNAs as biomarkers of lung cancer

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460698
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kyorin University
• Principal Investigator: AISO TOSHIKO 杏林大学, 保健学部, 教授 (40195144)
• Co-Investigator(Renkei-kenkyūsha): GAMOU SHINOBU 杏林大学, CCRC研究所, 教授 (90122308) ; HIGASHI KATSUMI 杏林大学, 保健学部, 特任教授 (50159109)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: マイクロRNA / バイオマーカー / 肺がん / リアルタイムPCR

Outline of Final Research Achievements

We analyzed circulating microRNAs miR-20a, miR-21, miR-145 and miR-223 as candidate markers of lung cancer: (1) miR-145 was degraded rapidly in serum samples at 4°C; the level dropped to 29.3% over 24 h. MiR-145 in extracellular vesicles exhibited similar instability; the level was 56.5%; (2) The level of microRNA miR-21 in serum decreased 44% in healthy passive smokers. (3) On two of these four microRNAs, the levels decreased significantly in serum of non-small cell lung cancer patients. Moreover, these levels in serum increased after the excision of the cancer.

Free Research Field

分子生物学および臨床検査医学