The development of novel, quick, and simple FRET-based telomerase assay method

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460708
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Konan University
• Principal Investigator: MURASHIMA TAKASHI 甲南大学, フロンティアサイエンス学部, 教授 (20263923)
• Co-Investigator(Renkei-kenkyūsha): MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 教授 (50388758)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 凝集誘起発光 / テロメア / アミロイドβ / ガン / アルツハイマー病

Outline of Final Research Achievements

A simple and sensitive methods for measuring telomeric tandem repeat DNA and amyloid-β based on AIE phenomena were developed. According to these methods, telomere concentration can be estimated with high sensitivity (5 pM for 12-mer telomeric sequences) using AIE-CCCTAACCCTAA probe. On the other hand, the detection of Aβ was performed with AIE dye-amyloid fibrillization-promoting peptide (AFPP) probe. While Aβ was added to the probe solution, the aggregation of Aβ was promoted and the fluorescence was enhanced within 3 hrs. The limit of detection with this method is 4.2 nM.
This method provides a simple, rapid, sensitive and cost-effective assay for telomere sequence and Aβ.

Free Research Field

有機合成化学