Proteomic analysis of membrane vesicles for elucidation of tumor communication mechanism and identification of tumor markers

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460715
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: National Institutes of Biomedical Innovation, Health and Nutrition
• Principal Investigator: Kume Hideaki 国立研究開発法人医薬基盤・健康・栄養研究所, プロテオームリサーチプロジェクト, 研究員 (50322714)
• Co-Investigator(Kenkyū-buntansha): TOMONAGA Takeshi 独立行政法人医薬基盤研究所, プロテオームリサーチプロジェクト, プロジェクトリーダー (80227644) ; MATSUBARA Hisahiro 千葉大学, 医学系研究科, 教授 (20282486)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 癌 / プロテオミクス / 細胞外小胞

Outline of Final Research Achievements

We have previously performed quantitative proteomic analysis of membrane fractions prepared from colorectal cancer tissues and benign polyps for biomarker discovery. In this study, we investigated whether the biomarker candidates could be detected in extracellular vesicle (EV) fraction prepared from serum samples. The EV fraction was prepared from serum samples of controls and colorectal cancer patients with or without metastasis by ultracentrifugation. A total of 20 proteins were quantitated by selected reaction monitoring (SRM) and three of them were shown to be increased in cancer patients with metastasis compared with controls or cancer patients without metastasis. In addition, these proteins were significantly elevated even in early stages compared with control in an independent set of patient samples. Accordingly, these proteins may be useful tumor markers for the diagnosis of colorectal cancer.

Free Research Field

プロテオミクス