2016 Fiscal Year Final Research Report
Deveropement of nano-fludic technology based PCR for epidemiology of pneumococcus serotypes
| Project/Area Number |
25460761
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Epidemiology and preventive medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MORIMOTO Konosuke 長崎大学, 熱帯医学研究所, 准教授 (50346970)
DHOUBHADEL Bhim Gopal 長崎大学, グローバルヘルス研究科, 助教 (80761113)
SUZUKI Motoi 長崎大学, 熱帯医学研究所, 助教 (60444874)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 肺炎球菌 / 血清型 / マルチプレックスPCR |
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| Outline of Final Research Achievements |
Pneumococcus is one of the most important pathogens of pneumonia and meningitis over the world. It has around 100 types of serotype and pneumococcal vaccines covers 10 to 23 serotypes. To know epidemiological distribution of serotypes in carriage among children and pneumonia plays crucial roles in control of diseases by vaccines.We established new method of multiplex real-time PCR for 50 serotypes using nano-fluidic technology. We tested samples of naso-pharyngeal swab taken from children in Vietnam and sputum of adult community-onset pneumonia, and revealed that our new method was very useful for epidemiological research of pneumococcal diseases. We found that some part of healthy children and patients with pneumonia had more than one serotype. This is a novel finding and we will develop our research to investigate the role of these multi-serotypes infection or carriage in pneumococcal diseases.
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| Free Research Field |
感染症内科学
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