2015 Fiscal Year Final Research Report
The comprehensive analysis of microRNA using next generation sequencer (NGS) for forensic application
| Project/Area Number |
25460860
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | マイクロRNA / ラット / 次世代シークエンサー / 死後のストレスマーカー / 死後経過時間推定 |
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| Outline of Final Research Achievements |
The microRNAs (miRNAs) are known to be stable at postmortem because they are short-length (about 20 nucleotides) single-strand molecules. In this study, we searched miRNAs acting as biological markers to estimate the time since death or the level of stress before death by experiments using rats. Then the good correlations were observed between the postmortem time and levels of instable cytoplasmic RNAs, when miR-16 in heart, miR-21 in liver and miR-451 in blood were used as internal standards. In addition, miRNAs in rats under stresses of alcohol intake, running and suppression by water bag were analyzed by the next generation sequencer, levels of several miRNAs were found to be increased or decreased by the stresses.
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| Free Research Field |
法医学
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