2015 Fiscal Year Final Research Report
Rapid and sensitive determination of toxic substances simultaneously by MALDI mass spectrometry
| Project/Area Number |
25460867
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 質量分析 / MALDI / 金属 / アンフェタミン / 危険ドラッグ |
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| Outline of Final Research Achievements |
Nowadays various medicines and toxic materials are prevailed. Furthermore, undesignated but toxic synthetic substances have been appeared on illicit drug markets. Matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS) is one of the most sensitive MS methods that can detect molecules having wide range of molecular mass simultaneously. The MS data can be obtained within 20 s for each sample only by inserting 100 samples placed on a plate into the instrument. Using MALDI-MS we developed simultaneous detection methods for three groups of toxic substances, respectively; ① metals (Pt, Au, Cu, Mo, As), ② amphetamines (A, MA, MDA, MDMA), ③ synthetic cathinones (PPP, MPPP, PBP, PVP) , (PVT, F-PVP, MPHP, PV8, PV9, F-PV9). The methods were applied to the detection of toxic substances obtained from cadavers, respectively. Multi-analytes detection using a single sample contributes greatly in the forensic and clinical toxicology where toxic substances are sometimes unknown.
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| Free Research Field |
医歯薬学
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