Research for metabolites of methomyl -Focus to volatile compounds-

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460873
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: University of the Ryukyus
• Principal Investigator: FUKE Chiaki 琉球大学, 医学(系)研究科(研究院), 准教授 (60173374)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: メソミル / ジメチルジスルフィド / クロマトグラフ質量分析計 / ヘッドスペース分析法 / メソミル分解酵素 / 肝臓・腎臓 / 腸内細菌 / Bacillus cereus

Outline of Final Research Achievements

We analyzed specimens of a methomyl poisoning victim by head-space GC/MS, and dimethyl disulfide (DMDS) was detected. DMDS is an organic sulfur compound and has characteristic odor. However there are no reports associated methomyl with DMDS. In order to confirm that DMDS is a decomposed compound from methomyl, we conducted in vitro examination using fresh pork liver and methomyl. Fresh pork liver homogenate (w/w: 1:1 with saline) was incubated with methomyl at 37 degrees centigrade. The phenomenon which the methomyl concentration was decreased and the DMDS concentration was increased was observed. When fresh pork homogenate alone or methomyl only was incubated at same manner, DMDS was not detected.
DMDS was a decomposed compound from methomyl. We expect that the characteristic odor of DMDS is help to find a victim poisoned by methomyl.

Free Research Field

法医中毒学