Research on rapid identification method of pathogenic virus based on exhaustive gene amplification

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460882
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: National Research Institute of Police Science
• Principal Investigator: Muto Junji 科学警察研究所, 法科学第一部, 主任研究官 (80432186)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: ウイルス / 網羅的遺伝子検出法 / PCR / 死後経過 / 同定 / 定量PCR / 病理検査 / 法医学

Outline of Final Research Achievements

We explored detectability of infecting viral genes from forensic sample including swab and organ by applying rapid determination system of viral RNA/DNA sequences (RDV). We detected genes of feline calicivirus and feline herpesvirus from throat and nasal cavity swab samples of autopsied cat by the RDV method, and then we rapidly identified these viruses by PCR and direct sequence of PCR product. To measure the impact of postmortem change on viral genes contained in organ, we performed quantitative PCRs that amplify a variety of lengths of influenza virus gene. As the result, it suggested the possibility that influenza virus genes were broken in the lung sample of the infected mouse. Therefore, it was deemed desirable to use a comparatively short fragment amplified by the RDV method in the case of detection of virus gene from organ sample.

Free Research Field

ウイルス学