2015 Fiscal Year Final Research Report
The induction of efficient cardiac differentiation of human multipotent mesoangioblasts
| Project/Area Number |
25460917
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine(including psychosomatic medicine)
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIOJIMA Ichiro 関西医科大学, 医学部, 教授 (90376377)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 幹細胞動員 |
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| Outline of Final Research Achievements |
Very recently, we identified circulating mesoangioblasts (cMABs), a subset of mesenchymal stem cells that co-express cardiac mesodermal markers from the patients undergoing cardiac catheterization. Here, we explored #1: where cMABs come from and #2: how we can induce cardiac differentiation of cMAB efficiently.
#1. Fist, we examined patients undergoing cardiac catheterization. We simultaneously obtained blood samples from aortic sinus (Ao), coronary sinus (CS: drain of the blood perfusing heart) and right atrium (RA) and compared the number of obtained cMAB colonies among those three different points. We confirmed that the heart is predominant niche of human cMABs. For further confirmation, we planed 2 clinical and 1 experimental studies. However, these studies were stopped by unexpected situations. #2. We tested several medium and matrix. Finally cells (MABs) were quite similar to iPSCs after induction of cardiac differentiation, however we could not see beating MABs.
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| Free Research Field |
循環器内科学
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