2016 Fiscal Year Final Research Report
Development of novel therapy based on epigenetic alterations in Barrett's esophagus
| Project/Area Number |
25460923
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | バレット食道 |
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| Outline of Final Research Achievements |
We successfully established primary cell culture of patient-derived Barrett's esophageal cancer (BE ca) using conditioning reprogramming method. To investigate the carcinogenic mechanisms of BE ca, the primary cells were exposed to low pH bile acids. The exposures of low pH bile acid decreased mRNA expressions of p63 and CK5. It was speculated that the post-transcriptional regulations such as abnormal gene methylations or histone acetylations were involved in the altered expressions of these genes. However, treatments of demethylation or deacetylation did not change the expressions of these mRNA, indicating the other mechanisms are associated with the effects of the exposures of low pH bile acid. We are continuously analyzing the mechanisms. In parallel, our transcriptome analysis identified novel alterations of nine gene expressions which were epigenetically regulated in the process to develop BE ca. We are analyzing the roles of these gene alterations in the cancer development.
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| Free Research Field |
消化器内科
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