Role of enteric neurons in regulation of intestinal inflammation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460942
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Chiba University
• Principal Investigator: fujimura lisa 千葉大学, バイオメディカル研究センター, 助教 (30376363)
• Co-Investigator(Kenkyū-buntansha): HATANO Masahiko 千葉大学, 大学院医学研究院, 教授 (20208523)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 腸管神経 / 腸炎 / 腸内細菌叢 / 腸内細菌科

Outline of Final Research Achievements

Ncx-deficient (KO) mice exhibited increased number of enteric neuron (EN) and hyper sensitive to DSS induced colitis model. In this study, we characterized the fecal microbiota to examine whether increased number of EN affects enteric flora. CFUs of aerobic bacteria increased in the KO mice compared to those of wild type mice. Meta-genome analysis revealed that the family Enterobacteriaceae were significantly higher in the KO mice compared with the wild type mice. Since bacterial NO reductase gene V (NorV) which detoxificate NO, determine a virulence of bacteria, more NorV+ bacteria were detected from the feces of the KO mice compared to that of wild type mice. To elucidate whether the dysbiosis affect the severity of the DSS-induced colitis, fecal transfer experiments were carried out. Mice transferred with feces of the KO mice developed more severe colitis than wild type transferred one. Our study implies that EN participates in regulation of intestinal microbiota.

Free Research Field

分子生物学　発生工学