Bile acid and gut luminal environment modulates fatty-acid sensing by GPR40

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460950
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya University
• Principal Investigator: YAMAMOTO AKIKO 名古屋大学, 総合保健体育科学センター, 准教授 (60402385)
• Co-Investigator(Kenkyū-buntansha): ISHIGURO HIROSHI 名古屋大学, 総合保健体育科学センター, 教授 (90303651)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: GPR40 / 脂肪酸

Outline of Final Research Achievements

The presence of ingested fat in the proximal small intestine induces secretion of CCK from a type of enteroendocrine cells: I-cells. It is now clear that GPR40 is the functional sensor of FFAs of CCK cells (I cells). However, we still need to clarify (1) the precise structural requirements (size of aggregates or micelles, etc.) of FFAs as effective ligands of GPR40 and (2) the roles of gut luminal environment for proper fatty-acid sensing. In this study, to clarify the cellular mechanisms by which bile acids and gut luminal environment influence fatty-acid sensing mechanisms, we examined the combined effects of fatty acids and bile acids on [Ca2+]i in PC12 cells expressing GPR40.

Free Research Field

膵臓、消化管