2015 Fiscal Year Final Research Report
Identification of the pathogenic virus of ulcerative colitis using the next-generation sequencing techonology
| Project/Area Number |
25460961
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Medical and Dental University (2014-2015)
Keio University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 潰瘍性大腸炎 / 次世代シーケンサー / ウイルス |
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| Outline of Final Research Achievements |
The cause of ulcerative colitis is unclear. Because the next-generation sequencer can determine nucleotide sequence without needing known base sequence information, it is used for identification of unknown pathogenic viruses. In this study, an unknown virus is assumed as the etiology of ulcerative colitis and was intended to identify this.
Colonic mucosal samples were obtained from patients with ulcerative colitis. Nucleotide sequence was determined with a next-generation sequencer. Human and bacterial nucleotide sequence was removed and virus nucleotide sequence was extracted on homologous analysis. More than 300 virus sequences per 1 sample were identified. The most frequently identified sequences were those similar to Human endogenous retrovirus, and Stealth virus.
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| Free Research Field |
医歯薬学
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