2015 Fiscal Year Final Research Report
The Mechanism of disintegration and redifferentiation of cell adhesion proteins through CDX2 in chemotherapy refractory colorectal cancer.
| Project/Area Number |
25460962
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University |
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Principal Investigator |
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| Research Collaborator |
LYNCH JOHN P University of Pennsylvania, Department of Medicine, Division of Gastroenterology, Associate Professor of Medicine
AZUMA TOSHIFUMI 東京歯科大学, 歯学部, 教授 (00222612)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | CDX2 / SP細胞 / 細胞接着関連タンパクの破綻 / 化学療法抵抗性大腸癌 |
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| Outline of Final Research Achievements |
Generating pHT- control, pHT-Cdx2, and pHT-Cdx2-ShRNA-infected Colon cancer cells, the phosphorylation states of several receptor tyrosine kinases and the expression of cell adhesion marker were analyzed. Side population cells derived from each cancer cells were investigated in terms of cell function, proliferation, adhesion and invasion potential. Using spheroid-based drug screen, we are determined to pursue the mechanism of disintegration and redifferentiation of cell adhesion proteins through CDX2 in chemotherapy refractory colorectal cancer. Especially, we focus on the mechanism the regulation of Rac1 expression by Cdx2.
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| Free Research Field |
Oncology
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