Primary tumor culture for personalized medicine

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Center for Clinical and Biomedical Research, Sapporo Higashi Tokushukai Hospital
• Principal Investigator: Ashida Toshifumi 医療法人徳洲会札幌東徳洲会病院付属臨床研究センター, その他部局等, 研究員 (50261409)
• Co-Investigator(Kenkyū-buntansha): MIZUKAMI Yusuke 医療法人徳洲会 札幌東徳洲会病院, 付属臨床研究センター 臨床生体情報解析部, 部門長 (30400089)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 初代培養

Outline of Final Research Achievements

The application of primary cultures preserving intrinsic property of cancers holds promise for serving human resources to uncover core pathway guiding novel therapy and to provide patient-specific tumor antigens. In the current study, we sought to develop a culture system that can allow pancreatic neoplasia at various stages to adapt in vitro microenvironment without losing tumor forming capability in vivo. The primary culture by combining tumor oraganoid isolation and conditional reprogramming facilitated to rebuild physiological tumor structure by transplanting to immunodeficient mice, whereas most commercially available cell lines did not. Sequencing data of the primary culture indicated these tumor cells include heterogeneity of the primary tumors, providing useful model to learn clonal evolution of the precursors. These studies demonstrate the general utility of a tractable tumor culture for human cancer modeling and therapeutic strategy against pancreatic neoplasms.

Free Research Field

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