2015 Fiscal Year Final Research Report
Development of new antiangiogenic therapy specific for tumor tissue of hepatocellular carcinoma with microRNA
| Project/Area Number |
25461018
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中村 徹 久留米大学, 医学部, 助教 (30341332)
古賀 浩徳 久留米大学, 医学部, 教授 (90268855)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 肝細胞癌 / 内皮細胞 / 血管新生抑制療法 / マイクロRNA / 血管新生 |
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| Outline of Final Research Achievements |
We extracted mRNA and microRNA from endothelial cells in HCC tissues. The expression of twelve mRNAs related angiogenesis was upregulated. The expression of miR-127-3p, miR-379-5p and miR-711 was upregulated and 70 microRNAs were downregulated in HCC tissues. miR30a-5p which was the most downregulated was added to cultured endothelial cells from HCC tissues. The growth activity of endothelial cells from HCC tissues was suppressed by miR30a-5p addition. In vivo experiments, tumor growth of HCC was suppressed by miR30a-5p addition from mouse tail vein. Tumor angiogenesis was suppressed in HCC tissues by miR30a-5p addition. However, sinusoidal density was not reduced by miR30a-5p.
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| Free Research Field |
医歯薬学
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