Non-invasive modality for diagnosis of pancreatic neoplasm

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Center for Clinical and Biomedical Research, Sapporo Higashi Tokushukai Hospital
• Principal Investigator: Mizukami Yusuke 医療法人徳洲会札幌東徳洲会病院付属臨床研究センター, その他部局等, その他 (30400089)
• Co-Investigator(Kenkyū-buntansha): ASHIDA Toshifumi 医療法人徳洲会 札幌東徳洲会病院, 付属臨床研究センター IBD研究部, 研究員 (50261409) ; MORIICHI Kentaro 旭川医科大学, 医学部 内科学講座, 講師 (70455715)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 膵癌 / 低侵襲診断

Outline of Final Research Achievements

Recent technologies are emerging to allow genotyping of plasma cell-free DNA (cfDNA) shed from tumors into the general circulation. There are two main types of pathologically distinct precursors for PDA, pancreatic intraepithelial neoplasias and intraductal papillary mucinous cystic neoplasias (IPMNs), and the mutations in KRAS and/or GNAS mediate key signaling during early development of the tumors. The clinical viability of cfDNA genotyping using digital PCR targeting mutations in KRAS and GNAS were examined. Ten PDA and 21 IPMN patients were recruited. The primary endpoint of this study was to evaluate whether such an approach can appropriately monitor the risk IPMN progression and detect localized early-stage PDA non-invasively. The major technical challenge of widespread implementation of this clinical test is the coverage for uncommon forms of KRAS mutation, as well as sensitivity for early stages cancer where the amount of cfDNA may be limited.

Free Research Field

消化器病