2015 Fiscal Year Final Research Report
Development of PI Polyamide targeting adiponectin gene
| Project/Area Number |
25461092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UENO Takahiro 日本大学, 医学部, 兼任講師 (40386008)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | PI ポリアミド / アディポネクチン |
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| Outline of Final Research Achievements |
Pyrrole-Imidazole polyamides (PI polyamides) are new chemical compounds that specifically inhibit transcription factor binding. A PI polyamide that targets mouse adiponectin(APN) and increases APN gene expression was designed.
Treatment of 3T3-L1 cells with 1.0μM PI polyamide significantly increased APN mRNA expression. PI polyamide administration significantly improved blood glucose level during the glucose tolerance test in db/db mouse. PI polyamide treatment also improved insulin sensitivity in db/db mouse evaluated by the insulin loading test. PI polyamide significantly decreased plasma triglyceride and free fatty acid levels in db/db mouse. Moreover, lipid deposits of the liver in db/db mouse were decreased by PI polyamide administration.The designed PI polyamide targeting SPN gene successfully increased APN expression, and improved insulin sensitivity in the diabetic mouse model. This novel compound is promising as a useful compound in the treatment of diabetes.
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| Free Research Field |
代謝
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