Excess Salt Increases Infarct Size Produced by Photothrombotic Distal Middle Cerebral Artery Occlusion in Spontaneously Hypertensive Rats

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461098
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: National Hospital Organization, Hizen Psychiatric Center
• Principal Investigator: Yao Hiroshi 独立行政法人国立病院機構肥前精神医療センター(臨床研究部), その他部局等, その他 (20265010)
• Co-Investigator(Kenkyū-buntansha): NABIKA Toru 島根大学, 医学部 病態病理学, 教授 (50180534)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 過剰な食塩摂取 / 高血圧 / 脳梗塞 / 局所脳虚血モデル / 高血圧自然発症ラット / 血液脳関門 / 脳血流

Outline of Final Research Achievements

Cerebral circulation is known to be vulnerable to high salt loading. After 14 days of salt loading (0.9% saline), spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO). After MCAO, regional cerebral blood flow (CBF), determined with laser-Doppler flowmetry, was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p=0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.

Free Research Field

脳血管障害