The role of ADAMTS in cardiovascular disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Okayama University
• Principal Investigator: KUSACHI Shozo 岡山大学, 保健学研究科, 特命教授 (30214943)
• Co-Investigator(Kenkyū-buntansha): HIROHATA Satoshi 岡山大学, 大学院保健学研究科, 教授 (90332791) ; OGAWA Hiroko 岡山大学, 大学病院, 助教 (70423283)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 心臓病学 / ＥＣＭ

Outline of Final Research Achievements

The aim of this study was to examine the role of ADAMTS in cardiovascular disease. We used ADAMTS knockout mouse in this study. First, we produced ADAMTS4/ADAMTS5 double knockout mouse. Because ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2) share the substrates, one of these two molecule may compensate the biological function in a single knockout mouse, we decided to produce double knockout mouse.
The double knockout mice were survived and there was no dwarfism observed. There was no particular abnormality in the tissue development. We then cross double knockout mice with ApoE knockout mouse. ApoE knockout mouse is used for atherosclerosis study. We succeeded to produced triple knockout mice and we gave them high-fat diet. It was interesting that there was a significant difference in the development of atherosclerosis between ApoE single and triple knockout mice.

Free Research Field

循環器病学