Elucidation of intrinsic fibrinolytic activities potentiated vascular endothelial cells and its possibility in prophylaxis of thrombotic disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Suzuki Yuko 浜松医科大学, 医学部, 准教授 (20345812)
• Co-Investigator(Kenkyū-buntansha): URANO TETSUMEI 浜松医科大学, 医学部, 教授 (50193967) ; SANO HIDETO 浜松医科大学, 医学部, 助教 (80623842)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 血管内皮細胞 / 線溶活性 / 組織型プラスミノゲンアクチベータ / リアルタイムイメージング解析 / 蛍光顕微鏡

Outline of Final Research Achievements

Vascular endothelial cells (VECs) play essential role in keeping the fluidity of circulating blood by expressing dynamic antithrombotic effects. Tissue-type plasminogen activator (tPA) secreted from VECs is a key molecule for initiating fibrinolytic, and retained on the cell surface after secretion. Cell surface-retained tPA effectively evokes both plasminogen activation and fibrin clot dissolution (fibrinolysis) on VECs. Our quantitative assessment of these two different plasminogen activator activities contributes to identify the unique characteristics of TNK-tPA (tenecteplase) having lower affinity to VEC surface compared to wild-type (alteplase).

Free Research Field

細胞生理学