The effect of SRT1720 on HSP47 expression in murine bleomycin-induced pulmonary fibrosis

2016 Fiscal Year Final Research Report

• Project/Area Number: 25461183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: University of Toyama
• Principal Investigator: YAMADA Toru 富山大学, 大学院医学薬学研究部(医学), 特命助教 (40512091)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 細胞・組織 / 呼吸器学 / 肺線維症 / 熱ショック蛋白

Outline of Final Research Achievements

In lung fibroblast CCD33Lu cells, simultaneous stimulation of TGF-beta1 and SRT1720 did not enhance the expression of HSP47 in comparison with single stimulation of TGF-beta1.However, pre-incubation of SRT1720 two hours before enhanced the expression of HSP47 mRNA. Immunohistochemistry could not show the significant difference of the expression of HSP47 in murine bleomycin-induced pulmonary fibrosis between SRT1720 treated group and untreated group. Although SRT1720 enhanced the expression of HSP47 mRNA, HSP47 did not increase in protein levels. We could not conclude that SRT1720 increased the pulmonary fibrosis via HSP47 expression.

Free Research Field

（呼吸器病学）