Effect of respiratory disease onset and functionality in the alveolar-bronchial of oxidative stress defense protein DJ-1

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Toho University
• Principal Investigator: TAIRA Takahiro 東邦大学, 医学部, 教授 (70197036)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 酸化ストレス / DJ-1タンパク質 / 慢性閉塞性肺疾患 / 薬物輸送担体

Outline of Final Research Achievements

I examined the involvement of the function of the lungs and oxidative stress defense protein DJ-1 respiratory disease onset. Using human lung epithelial cell-derived A549, to establish a DJ-1 protein knockdown cell line. As a result, the knockdown cell lines, became more vulnerable to oxidative stress. Then, the gene whose expression is varied among A549 cells and knockdown cell lines were screened by DNA micro array. As a result, gene expression of some transporters were varied. The transporter involved in respiratory disease onset have been reported. The transporter and DJ-1 are co-localization is revealed from confocal microscopy. Coordinated action of DJ-1 protein and the transporter was suggested.

Free Research Field

分子生物学、細胞生物学