Analysis of T helper (Th) responses in experimental peritoneal fibrosis with Th1/Th2/Th17 dominate mice

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: Yoh Keigyou 筑波大学, 医学医療系, 研究員 (90323302)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 被嚢性腹膜硬化症 / Tヘルパー細胞 / Th1細胞 / Th2細胞

Outline of Final Research Achievements

Encapsulating peritoneal sclerosis (EPS) is one of the most serious complications of long-term peritoneal dialysis. The purpose of this study was to clarify the roles of T helper (Th) (Th1/Th2/Th17) cells in the progression of peritoneum fibrosis (PF) in EPS. T-bet, GATA-3, and RORγt are Th1, Th2, and Th17 lineage commitment transcription factors, respectively. In this study, Th1-biased (T-bet transgenic (Tg)) mice, Th2-biased (GATA-3 Tg) mice, and Th17-biased (RORγt Tg), and wild-type mice were administered chlorhexidine gluconate (CG) intraperitoneally. CG-injected GATA-3 Tg mice developed marked PF. In contrast, CG-injected T-bet Tg mice only developed mild PF. Cytokines analysis in peritoneal fluid showed that IFN-γ was significantly increased in CG-injected T-bet Tg mice. Moreover, intraperitoneal administration of IFN-γ improved PF in GC-injected wild-type mice. Our results suggest that the regulation of Th cells and cyotokines may alleviate the severity of experimental PF.

Free Research Field

腎臓内科学