2015 Fiscal Year Final Research Report
Role of microRNA targeting PTN and CTGF in peritoneal dialysis
| Project/Area Number |
25461246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Nara Medical University (2015)
Kyoto University (2013-2014) |
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Principal Investigator |
Kasahara Masato 奈良県立医科大学, 医学部, 病院教授 (50393351)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOI HIDEKI 京都大学, 医学研究科, 助教 (90378779)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 腹膜透析 / microRNA / マウス / トランスフェクション / 培養細胞 |
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| Outline of Final Research Achievements |
microRNA regulates protein transcription of the targeted gene. We investigated the role of miRNA using peritoneal fibrosis mice induced by intraperitoneal injection of chlorhexidine gluconate (CG), and showed the increase of miR-26a and miR-30c both of which target CTGF and of miR-93 targeting VEGF. Administration of PDGF-BB or Angiotensin II induced miR-26a and miR-30c expression in cultured mesothelial cell line MeT5A. We further demonstrated the amelioration of peritoneal fibrosis induced by CG in tamoxifen-inducible systemic CTGF conditional knockout mice.
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| Free Research Field |
腎臓
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