2015 Fiscal Year Final Research Report
The development of in vivo gene delivery systems to peritoneum to inhibit peritoneal fibrosis
| Project/Area Number |
25461252
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 腹膜線維症 / 遺伝子治療 / ドラッグデリバリー / スモールRNA |
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| Outline of Final Research Achievements |
1.We developed an in vivo siRNA delivery system with liposome based nanoparticles (NPs) to peritoneum to inhibit peritoneal fibrosis. TGF-β1-siRNAs encapsulated in NPs (TGF-β1-siRNAs-NPs) dissolved in peritoenal dialsysis (PD) fluid were intraperitoneally injected into the peritoneal fibrosis (PF) mice. They significantly knocked down TGF-β1 expression in peritoneum and inhibited peritoneal thickness with fibrous change.
2. We identified microRNA(miRNA)-21-5p inhibition by anti-miRNA-21-5p-LNA is effective for the prevention of PF. We also identified that sevreal microRNAs (miRNA-21-5p, miRNA-221-3p, miRNA-34a-5p and miRNA-327) may be useful as novel diagnostic biomarkers for PF.
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| Free Research Field |
ドラッグデリバリー
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