2015 Fiscal Year Final Research Report
Functional analysis of pancreatic beta-cells in metabolic disorders using the fluorescent ATP probe gene transgenic mouse
| Project/Area Number |
25461346
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
NAGASHIMA KAZUAKI 京都大学, 医学(系)研究科(研究院), 講師 (40324628)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IMAMURA HIROMI 京都大学, 生命科学研究科, 准教授 (20422545)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | ATP / KATPチャネル / 糖代謝異常 |
|---|
| Outline of Final Research Achievements |
To visualize and analyze intracellular ATP dynamics in physiological condition, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP. Using the fluorescent ATP probe, the fluorescent Ca2+ probe, and the transgenic mouse expressing ATP probe protein in pancreatic beta-cells, we carried out several functional analyses of intracellular ATP and Ca2+ concentrations in living pancreatic beta-cells.
|
| Free Research Field |
医歯薬学
|
