2015 Fiscal Year Final Research Report
The role of prolyl isomerase Pin1 in obesity
| Project/Area Number |
25461355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Hiroshima University |
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Principal Investigator |
Nakatsu Yusuke 広島大学, 医歯薬保健学研究院(医), 助教 (20452584)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | Pin1 / AMPK / 肥満 |
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| Outline of Final Research Achievements |
In this study, we investigated the role of prolyl ismoerase Pin1 in obesity . We identified AMPK gamma subunit as a Pin1 binding parter. Pin1 overexpression decreased AMPK phosphorylation by 2-deoxy-glucose, while Pin1 knockdown by siRNA enhanced. Pin1 canceled the protective effect of AMP on dephosphorylation, resulting in inducing the decrease of AMPK phosphorylation.
Lastly, we examined the phosphorylation levels of AMPK in mice muscle. We found Pin1 deficiency in muscle enhanced AMPK phosphorylation.
In summary, Pin1 regulates lipid metabolisn through decreasing AMPK phosphorylation.
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| Free Research Field |
代謝学
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