Elucidation of mechanism that regulate energy metabolism and food intake by the identification of new bioactive peptides

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Yoshida Morikatsu 国立研究開発法人国立循環器病研究センター, 研究所, 上級研究員 (70393212)
• Co-Investigator(Kenkyū-buntansha): MIYAZATO MIKIYA 国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50291183)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生理活性ペプチド / オーファン受容体 / 摂食・エネルギー代謝調節

Outline of Final Research Achievements

We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey). Using this system, four peptides that increase impedance change of the cells expressing a certain orphan receptor were isolated from tissue extracts. At a result of sequence analysis, these peptides are fragment from four different proteins. These peptides increased not only cellular impedance change but also intracellular Ca2+ concentration. Compared with intracellular Ca2+ detection system, CellKey showed sensitive detection system. This system is useful for future screening strategy.

Free Research Field

内分泌