The analysis of bone marrow microenvironment in myelodysplastic syndrome using patient-derived iPS cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461418
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Osaka University
• Principal Investigator: EZOE Sachiko 大阪大学, 医学部附属病院, 講師 (90379173)
• Co-Investigator(Kenkyū-buntansha): KANAKURA Yuzuru 大阪大学, 大学院医学系研究科, 教授 (20177489) ; SHIBAYAMA Hirohiko 大阪大学, 大学院医学系研究科, 講師 (60346202) ; ORITANI Kenji 大阪大学, 大学院医学系研究科, 准教授 (70324762) ; MIZUKI Masao 大阪大学, 医学部附属病院, 准教授 (80283761)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: iPS細胞 / 骨髄異形成症候群 / 疾患特異的 / 骨髄微小環境

Outline of Final Research Achievements

The myelodysplastic syndrome(MDS) is characterized as the expansion of abnormal hematopoietic stem cell clone. For the purpose of exaggerating the interaction between hematopoietic stem cells and bone marrow microenvironment in MDS patients, we established iPS cell lines derived from MDS patients bone marrow cells. We observed some abnormalities in differentiation and morphology of hematopoietic cells differentiated from disease specific iPS cells having 7-kariotype, and we also detected morphological abnormalities in normal hematopoietic cells co-cultured with MSCs derived from patient specific iPS cells, which suggests that abnormal stromal cells in MDS might have some important roles in the pathology of MDS.

Free Research Field

血液内科学