The role of microRNA142 overexpression in B-cell tumor development

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Wakayama Medical University
• Principal Investigator: SONOKI TAKASHI 和歌山県立医科大学, 医学部, 教授 (30382336)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: B細胞性腫瘍 / 染色体転座 / マイクロRNA

Outline of Final Research Achievements

(Summary) We performed functional analyses of miR142 using two mouse models. As a result, miR142 overexpression was found to cause a decrease in blood cells. (Result) (1) Bone marrow transplantation model: MiR142 and the GFP genes were introduced into bone marrow cells. The genes-introduced bone marrow cells were transplanted into the bone marrow-disrupted mouse. As a result, the blood cells which strongly express the miR142 were reduced. (2) Genetically modified model: Genetically modified mouse to be strongly expressed in B cells was generated. In genetically modified mice that were older than one year of age, IgM-positive cells were reduced compared to the wild type. (Conclusion) As mentioned above, our results suggest that miR142 overexpression is involved in the cancer development through disruption of cell fate.

Free Research Field

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