Development of the diagnostic marker in mucormycosis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461516
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Osaka City University
• Principal Investigator: Kakeya Hiroshi 大阪市立大学, 大学院医学研究科, 教授 (40398152)
• Co-Investigator(Kenkyū-buntansha): MIYAZAKI Yoshitsugu 国立感染症研究所, 真菌部, 部長 (00311861) ; SHIBUYA Kazutoshi 東邦大学, 医学部, 教授 (20196447) ; KOHNO Shigeru 長崎大学, 医歯薬学総合研究科, 客員研究員 (80136647)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ムーコル症 / 早期診断

Outline of Final Research Achievements

The diagnosis of mucormycosis was made by the identification of organism in the histopathology with culture confirmation. However, culture often yields no growth, and histopathological identification of organism with typical of Mucorales is sometimes difficult. Therefore, the reliable new diagnostic tool is expected. Secreted and cell-surface molecules are as target antigens for diagnostic tools. The signal sequence trap (SST) is a strategy to identify signal sequence containing cDNAs that encode secreted and cell-surface proteins. We constructed a SST c DNA library from RNA extraction from the clinical isolate Rhizopus oryzae. The ELISA kit to detect the target antigen was made and evaluated. The antigen A was also detected in R. oryzae infected mice sera. The 23kDa of the hypothetical protein, Antigen A, is a potential novel diagnostic maker in mucormycosis.

Free Research Field

感染症