DNA vaccine production fundamental Study based on the immune response analysis by group A hemolytic streptococcus held various pathogenic factor

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461519
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Kitasato University
• Principal Investigator: ARAI KAZUAKI 北里大学, 生命科学研究所, 講座研究員 (30547386)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Natuo 福島県立医科大学, 医学部, 准教授 (50466562) ; TAKAHAZHI Takashi 北里大学, 感染制御科学府, 教授 (00292855)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: A群溶血性連鎖球菌 / サイトカイン / ケモカイン / 病原性 / 炎症 / CpG-ODN / DNAワクチン

Outline of Final Research Achievements

Group A hemolytic streptococcus shows a fulminant streptococcal infection and a variety of pathological conditions become severe from mild pharyngitis. We focus on its various pathogenic agents, to purify the protein fraction containing the causative agent of the particular strain, and it is differentiated cells collected from mouse bone marrow macrophages GM-CSF, dendritic cells cells allowed to act on. A variety of cytokines and chemokine mRNA released from the cells by qRT-PCR, also detected the production of protein from both sides by the EIA method using the fluorescent magnetic microbeads. As a result, TNF-α, IFN-γ, IL-1β, IL-12p40, KC (CXCL2) significantly also IL-6, iNOS expression in viable cell, KC, elevated MIP-2 is a inflammatory cytokine It was also observed.

Free Research Field

感染症内科学