An analysis of carbapenem-inactivating effects of human cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 25461525
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Takemura Hiromu 聖マリアンナ医科大学, 医学部, 教授 (80301597)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 細胞 / カルバペネム系抗菌薬 / 不活化 / L-cysteine / A549

Outline of Final Research Achievements

At the study supported by JSPS KAKENHI Grant Number 22591114 from 2012 to 2014, we reported that antimicrobial activities of several carbapenems (Cps) decreased in the supernatants of human alveolar epithelial cell line A549.
In this study, the metabolomics analysis by mass spectrometry of the culture supernatants revealed that they contained L-cysteine (L-cys). We investigated how L-cys derived from A549 cells causes Cps inactivation by examining the correlation between the IPM-inactivating effects (CIE) of the A549 supernatants and the concentration of L-cys. The A549 culture supernatants exhibited CIE according to their L-cys concentrations and FCS inhibited the production of L-cys and CIE. The amino acids in the medium were necessary for the effects, so it is speculated that L-cys derived from the cells was a major potent contributor to the effects. Further studies were necessary to reveal the same inactivation occurs among the cells in the human body.

Free Research Field

臨床微生物学、感染症学、感染制御学、特に宿主・微生物・抗菌薬の関係性の実験的な評価を専門とする。