Development of diagnostic methods for all genotypes of norovirus.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461528
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Shirato Haruko (堀越東子) 国立感染症研究所, その他部局等, 研究員 (60356243)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ノロウイルス / 血液型抗原 / 診断 / 検出

Outline of Final Research Achievements

I attempted to establish two analytical methods to evaluate the carbohydrate-norovirus (NoV) interaction: surface plasmon resonance by using the virus-like particles (VLPs) of 25 strains from 18 genotypes and assays using red blood cells or cells modified on the glycosyltransferase gene. Then, using these analytical systems, I aimed at identifying the carbohydrate epitopes important in recognition by NoV and the carbohydrate-binding site on the virus. In addition, I analyzed the amino acid sequences of 15 genotypes, except the 18 genotypes used for this analysis, and narrowed down the candidate sequence of the carbohydrate-binding domain common to all 33 genotypes.

Free Research Field

ウイルス学