Detection of molecules involved in the induction of immunosuppressive function of MDSC-like cells induced by liposome

2016 Fiscal Year Final Research Report

• Project/Area Number: 25461578
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Asahikawa Medical College
• Principal Investigator: AZUMA Hiroshi 旭川医科大学, 医学部, 教授 (00167909)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: リポソーム / MDSC / immunosuppression / liposome / ナノ粒子

Outline of Final Research Achievements

Myeloid-derived suppressor cell (MDSC) appears in various pathological condition and suppress T cell immune response. Although cell-to-cell contact between MDSC and T cell is required for the suppression, the molecules involved in the contact remains unknown. When liposome particles are intravenously　injected into rat, Considerable amount of MDSC-like cells (liposome-internalized cells) can rapidly　accumulate in spleen. We further addressed its ability to produce cytokine. At the same time, tried to search the molecules involved in the cell-to-cell contact. Like MDSC, MDSC-like cells produce more IL-10 than normal macrphage. DNA microarray analysis revealed that B7-H3 message was amplified and cell surface expression B7-H3 was enhanced. Therefore, it is suggested that liposome can induce MDSC-like cells in vivo and B7-H3 molecules might be involved in T cell suppression.

Free Research Field

免疫学