2015 Fiscal Year Final Research Report
Suppression of food allergy by modulating autophagy function
| Project/Area Number |
25461584
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 食物アレルギー / オートファジー / 動物モデル |
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| Outline of Final Research Achievements |
Background: Autophagy is a fundamental bulk degradation process of cellular components. Autophagy is also associated with immune response. Therefore we analyzed the role of autophagy in the pathophysiology of food allergy. Methods: Balb/c mice were sensitized with Ovalbumin(OVA)/Alum and then were repetitively challenged with OVA. The effects of autophagy inhibitor, 3-methyladenine (3-MA) on allergic symptoms, mucosal mast cell protease-1 (mmcp-1), and OVA-specific cytokine producing ability of mesentric lymph node（MLN）cells, and mRNA expression levels in intestines were analyzed. Results: 3-MA treatment suppressed allergic symptoms induced by OVA challenge. In vitro OVA-specific Th2 cytokine production by MLN cells purified from 3-MA-treated mice were decreased compared with those of non-treated mice. 3-MA decreased the elevation of serum mmcp-1 levels and the expression levels of Th2 cytokine mRNA in the intestines.
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| Free Research Field |
アレルギー
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