A new trial of moleular genetics for the onset of Kawasaki disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461629
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Wakayama Medical University
• Principal Investigator: Suzuki Hiroyuki 和歌山県立医科大学, 医学部, 准教授 (80196865)
• Co-Investigator(Kenkyū-buntansha): Yoshikawa Norishige 和歌山県立医科大学, 医学部, 臨床研究センター長 (10158412) ; Takeuchi Takashi 和歌山県立医科大学, 医学部, 講師 (10246522) ; Suenaga Tomohiro 和歌山県立医科大学, 医学部, 助教 (70433365) ; Kakimoto Nobuyuki 和歌山県立医科大学, 医学部, 助教 (90614412)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 川崎病 / スーパー抗原 / レンサ球菌 / gene transfer

Outline of Final Research Achievements

In order to investigate whether superantigens (SAgs) are involved in the development of Kawasaki Disease (KD) by examining SAg genes in patients with KD, throat specimens were obtained from a total of 34 KD patients who fulfilled diagnostic criteria on admission. These samples were cultured with liquid culture solution of Brain-Heart Infusion Broth under 37℃ during overnight. Total DNA was extracted from these cultured bacterial solution. Using polymerase chain reaction, we examined the genes of five SAgs: streptococcal pyrogenic exotoxin-A (SPE-A), SPE-C, SPE-G, SPE-J, and Toxic Shock Syndrome Toxin-1. SPE-G, SPE-A, and SPE-J genes were detected in 3, 4, 8 specimens from KD patients. Two SAg genes of SPE-G and SPE-J were detected in one patients of KD. Now, we continue the examines for the identification of bacterial species of bacteria keeping SAg genes.

Free Research Field

小児循環器病、川崎病