Evaluation of Helicobacter hepaticus infection in children with liver disorders and postoperative biliary atresia

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461635
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Hyogo Medical University
• Principal Investigator: Okuda Masumi 兵庫医科大学, 医学部, 教授 (40531091)
• Co-Investigator(Kenkyū-buntansha): KAMIYA Shigeru 杏林大学, 医学部, 教授 (10177587) ; KIKUCHI Shogo 愛知医科大学, 医学部, 教授 (40224901) ; FUKUDA Yoshihiro 兵庫医科大学, 医学部, 名誉教授 (60148640) ; OSAKI Takako 杏林大学, 医学部, 准教授 (90255406) ; KONDOU Hiroki 大阪大学, 医学(系)研究科(研究院), 研究員 (10373515)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ヘリコバクター・ヘパティカス / 小児 / 肝障害 / イムノブロット / 抗体測定

Outline of Final Research Achievements

Helicobacter hepaticus (H. hepaticus) is a gram-negative, spiral shaped microaerophilic bacterium. Several studies have suggested the possibility that infection of this bacterium plays a role in the pathogenesis of liver diseases in humans. We evaluated the association between H. hepaticus and children with liver disorders. Furthermore, we detect the antigenic H. hepaticus protein recognized by child sera using 2-DE immunoblot analysis. Positive rate of H. hepaticus in children without and with liver disorders was 30.3% (10/33) and 44.4% (4/9), respectively. Total 25 proteins were identified as candidate antigens by LS-MS/MS after the 2-DE immunoblot analysis. Among the proteins, flagelin A was the most reactive protein, particularly against highly reactive sera, although low reactive sera also detected the spot slightly.

Free Research Field

感染症学　小児科学