Neuronal replacement therapy for neonatal hypoxic sichemiac brain injuty by exogenous bFGF and EGF administration

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461649
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kumamoto University
• Principal Investigator: IWAI MASANORI 熊本大学, 医学部附属病院, 講師 (80467993)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 新生児低酸素性虚血性脳症 / 内在性神経幹細胞 / EGF / ｂFGF / 脳虚血障害部位内投与 / 神経再生治療

Outline of Final Research Achievements

HI brain injury was induced in 7-days-old rat pups. Naive animals served as controls. EGF and bFGF were injected into the striatum and the cerebral cortex of the ischemic hemisphere at 3 days after HI. BrdU was injected intraperitoneally between 4 and 6 days after HI. Brains were removed and stored for immunehistological study. Cell proliferation was investigated by BrdU staining. Neural progenitor cells and matured neural cells were visualized with anti-DCX and anti-NeuN immunostaining using a microscope or confocal microscope, respectively. In the ischemic hemisphere at 7 days after HI, administration of EGF and bFGF combined significantly increased the number of BrdU positive cells in the SVZ compared to that in vehicle and naive controls, respectively. In the ischemic hemisphere with administration of EGF and bFGF, most BrdU positive cells were double positive for DCX. However, there is no significant change the number of BrdU+NeuN double positive cells among these three groups.

Free Research Field

新生児医学