Novel therapies for recessive dystrophic epidermolysis bullosa targeting at the COL7A1 promoter

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461681
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hokkaido University
• Principal Investigator: ARITA KEN 北海道大学, 医学(系)研究科(研究院), 客員研究員 (50374434)
• Co-Investigator(Kenkyū-buntansha): NOMURA Toshifumi 北海道大学, 北海道大学病院, 助教 (50399911) ; SHIMIZU Hiroshi 北海道大学, 医学(系)研究科(研究院), 教授 (00146672)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 表皮水疱症 / ７型コラーゲン

Outline of Final Research Achievements

No curative therapies for recessive dystrophic epidermolysis bullosa (RDEB) are currently available. In this study, we sought to develop novel therapies for this intractable disease targeting at the COL7A1 promoter. First, we constructed a vector containing the luc2P gene driven by the COL7A1 promoter and generated stable cell lines carrying the gene construct. Compound library screening using the stable cells identified a dozen of 'hits'. However, further screening showed that most of them are 'false-positive'. We are now testing these compounds using normal human epidermal keratinocytes to see if they up-regulate the COL7A1 promoter.

Free Research Field

皮膚科学