Functional analysis of IL-33 expressed in nucleus of normal human epidermal keratinocyte

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461709
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Jichi Medical University
• Principal Investigator: Tsuda Hidetoshi 自治医科大学, 医学部, ポスト・ドクター (30414923)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: IL-33 / 細胞周期 / 細胞分裂 / 細胞収縮環 / 表皮角化細胞 / siRNA

Outline of Final Research Achievements

IL-33 is expressed in the nucleus of epithelial and endothelial cells. In this study, it was investigated that the effect of IL-33 knockdown (KD) with siRNA in normal human epidermal keratinocytes (NHEKs). IL-33 KD attenuated BrdU uptake.The cell cycle analysis found that the number of cells in G2/M phase was increased in IL-33 KD cells.Immunofluorescence study revealed that there were many binucleated cells in IL33 KD cell culture, which were scarcely found in control cells. Western blot analysis revealed that IL-33 KD cells showed attenuated myosin light chain phosphorylation, which was reported to associate with contractile ring formation. Reduced expression of Ect2 was observed, which would have caused reduced activation of RhoA. These results suggest that nuclear IL-33 is positively involved in cell proliferation through promoting contractile ring formation by positively regulating myosin light chain phosphorylation, expression of Ect2, and activation of RhoA in NHEKs.

Free Research Field

皮膚科学　分子生物学　細胞生物学　遺伝学