Identification of molecules related to modulation of Amyloid beta production in human cerebrospinal fluid (CSF)

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461766
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: Osaka University
• Principal Investigator: Takashi Kodama 大阪大学, 医学(系)研究科(研究院), 特任研究員(常勤) (30512131)
• Co-Investigator(Kenkyū-buntansha): OKOCHI Masayasu 大阪大学, 医学研究科, 講師 (90335357) ; TAGAMI Shinji 大阪大学, 医学研究科, 助教 (40362735) ; YANAGIDA Kanta 大阪大学, 医学研究科, 特任研究員 (70467596)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アルツハイマー病 / 質量分析 / アミロイドベータ産生機構 / APL1ベータペプチド

Outline of Final Research Achievements

In this study, molecules in human cerebrospinal fluid (CSF) have been investigated using LC/MS/MS measurements and amino acid sequence search using public and home-developed databases. The purpose of this study is to establish a method to identify and quantify peptides which is related to the modulation of gamma-secretase and beta-secretase activities in Alzheimer’s disease (AD). We have identified 102 of membrane proteins, 54 of disease associated proteins, and 2 of neuro degeneration-related proteins in human CSF. We also developed and improved an advanced liquid chromatography tandem mass spectrometry (LC/MS/MS) method to determine the concentration of sub-femtomolar order of APLP1 derived peptides which is an established molecular marker of AD.

Free Research Field

生物物理学