Radiotoxixity after radioisotope therapy for bone metastases using gamma-H2AX foci of DNA danmage in lymphocytes

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461851
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kanazawa Medical University
• Principal Investigator: DOAI Mariko 金沢医科大学, 医学部, 助教 (40515673)
• Co-Investigator(Kenkyū-buntansha): WATANABE Naoto 金沢医科大学, 医学部, 教授 (40210926) ; IWABUCHI Kuniyoshi 金沢医科大学, 医学部, 教授 (10232696)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ストロンチウム / 放射線組織障害 / γ-H2AX / リンパ球

Outline of Final Research Achievements

The purpose of this study was to investgate the degree of radiotoxicity to lymphocytes following strontium-89 (Sr-89) therapy for bone metastases using γ-H2AX foci immunodetection. In addition, the degree of radiotoxicity to apoptosis of lymphocytes was also examined. This study was compared the γ-H2AX focus incidence in in vivo and in vitro irradiated lymphocytes to estimate the effective absorbed radiation dose to　lymphocytes in vivo. As a result, the radiation-induced DNA damage to the lymphocytes exposed to Sr-89 could be detectable using γH2AX foci immunodetection.
The apoptototic cells may also facilitate estimation of the radiotoxicity with this therapy. The γ-H2AX foci immunodetection and detection of apoptotic cells may facilitate estimation of the radiation doses absorbed and the degree of radiotoxicity to lymphocytes with this therapy

Free Research Field

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