Induction of functional exsomes selectively derived from mesenchymal stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461896
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: Tajima Katsushi 国立研究開発法人放射線医学総合研究所, 緊急被ばく医療研究センター, プログラムリーダー (80292423)
• Co-Investigator(Kenkyū-buntansha): YASUDA Takeshi 国立研究開発法人放射線医学総合研究所, 緊急被ばく医療研究センター, 主任研究員 (60332269) ; GOTOH Takaya 大東文化大学, スポーツ健康科学部, 教授 (80284355)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 間葉系幹細胞 / 再生医療 / エキソソーム / 放射線

Outline of Final Research Achievements

We investigated effects and mechanisms of mesenchymal stem cells (MSCs) against irradiated cells. Exosomes derived from MSCs inhibited cell-death of irradiated cells. Radiation increased cellular uptake of exosomes dependent on its doses, and induced CD29/CD81 complex formation on cellular surfaces. Exosomes were entered into cells through selectively binding its complex. The cellular uptake of exosomes were not dependent on phagocytosis, endocytosis, and pinocytosis. A factor “X” and exosomes from MSCs protected intestinal epithelial cell injury using radiation- exposure model animals. We demonstrated the mechanisms of MSCs on irradiated cells.

Free Research Field

放射線生物学